human il-18 elisa kit  (Multi Sciences (Lianke) Biotech Co Ltd)

Journal: American Journal of Translational Research

Article Title: AGTR1 inhibits pyroptosis and inflammatory cytokine secretion in rheumatoid arthritis fibroblast-like synoviocytes through the TGF-β pathway

doi: 10.62347/HTCE3264

Figure Lengend Snippet: AGTR1 regulates pyroptosis and inflammatory cytokine secretion. A, B. TUNEL staining of FLS treated with oe-NC, oe-NC+Nigericin, or oe-AGTR1+Nigericin. C, D. Flow cytometry analysis of caspase-1-positive cells. E-H. ELISA measurement of IL-1β, IL-18, IL-6, and IL-8 levels in culture supernatants. I. LDH release assay of FLS with different treatments. J. Representative images of dual immunofluorescence staining for PI (red) and Hoechst 33342 (blue).

Article Snippet: Secretion of IL-8 (E-EL-H6008), IL-18 (E-EL-H0253), IL-1β (E-EL-H0149), and IL-6 (E-EL-H6156) in RA-FLS supernatants was quantified using ELISA kits (Elabscience, China).

Techniques: TUNEL Assay, Staining, Flow Cytometry, Analysis, Enzyme-linked Immunosorbent Assay, Lactate Dehydrogenase Assay, Immunofluorescence

Journal: American Journal of Translational Research

Article Title: AGTR1 inhibits pyroptosis and inflammatory cytokine secretion in rheumatoid arthritis fibroblast-like synoviocytes through the TGF-β pathway

doi: 10.62347/HTCE3264

Figure Lengend Snippet: AGTR1 inhibits FLS pyroptosis and inflammatory cytokine secretion through the TGF-β pathway. A, B. WB detection of P-SMAD2, SMAD2, and TGF-β expression in FLS cells treated with oe-NC, oe-NC+Nigericin, and oe-AGTR1+Nigericin. C. The ratio of P-SMAD2 to total SMAD2 in FLS cells. D-F. WB detection of ASC, NLRP3, cleaved caspase-1, and GSDMD-N protein expression levels in RA-FLS cells under Nigericin induction after treatment with oe-NC, oe-AGTR1, and oe-AGTR1+SB505124 (TGF-β receptor I inhibitor). G, H. TUNEL staining to assess apoptosis in FLS cells after different treatments. I, J. Flow cytometry detection of caspase-1-positive FLS cells after different treatments. K-N. ELISA measurement of inflammatory cytokines IL-1β, IL-18, IL-6, and IL-8 levels in the supernatant of FLS cells after different treatments. O. LDH release assay of FLS with different treatments. P. Representative images of dual fluorescence staining with PI (red) and Hoechst 33342 (blue).

Article Snippet: Secretion of IL-8 (E-EL-H6008), IL-18 (E-EL-H0253), IL-1β (E-EL-H0149), and IL-6 (E-EL-H6156) in RA-FLS supernatants was quantified using ELISA kits (Elabscience, China).

Techniques: Expressing, TUNEL Assay, Staining, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Lactate Dehydrogenase Assay, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: Mogroside V Alleviates Renal Injury in Diabetic Mice via Regulation of the TLR4 / NF-κB Pathway and Modulation of ECM Remodeling

doi: 10.3390/ijms27146271

Figure Lengend Snippet: MV suppressed inflammatory responses. ( A ) The levels of IL-6, IL-18, IL-1β, TNF-α and MCP-1 in the supernatant were detected using ELISA after treatment with MV (25, 50, 100 mg/kg/d) or Met (101.4 mg/kg/d) in the serum of db/db mice (n = 3). ( B ) The levels of TNF-α, IL-1β, MCP-1 and IL-18 in the supernatant were detected using ELISA after treatment with HG in the presence or absence of MV or Met for 48 h in HRMC. ** p < 0.01 vs. respective control group; # p < 0.05 vs. respective DKD group (DKD mice for panel A, HG-treated HRMC for panel B); ## p < 0.01 vs. respective DKD group.

Article Snippet: ELISA kits for TNF-α (Cat: EK182), IL-1β (Cat: EK101B), IL-6 (Cat: EK106), MCP-1 (Cat: EK187), and IL-18 (Cat: EK118) were purchased from Hangzhou Lianke Biotech Co., Ltd. (Hangzhou, China).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Mogroside V Alleviates Renal Injury in Diabetic Mice via Regulation of the TLR4 / NF-κB Pathway and Modulation of ECM Remodeling

doi: 10.3390/ijms27146271

Figure Lengend Snippet: Effect of MV on inflammatory factors. ( A ) Western blot analysis was used to detect the expression of inflammatory proteins TNF-α, IL-1β, and IL-18 in db/db mice. ( B ) Western blot analysis was used to detect the expression of inflammatory proteins TNF-α, IL-1β, MCP-1 and IL-6 in HRMC. ** p < 0.01 vs. respective control group; # p < 0.05 vs. respective DKD group (DKD mice for panel A, HG-treated HRMC for panel B); ## p < 0.01 vs. respective DKD group.

Article Snippet: ELISA kits for TNF-α (Cat: EK182), IL-1β (Cat: EK101B), IL-6 (Cat: EK106), MCP-1 (Cat: EK187), and IL-18 (Cat: EK118) were purchased from Hangzhou Lianke Biotech Co., Ltd. (Hangzhou, China).

Techniques: Western Blot, Expressing, Control

Journal: Translational Cancer Research

Article Title: A pneumolysin mutant (ΔA146 ply) induces pyroptosis in triple-negative and HER2-positive breast cancer cells via the Caspase-1/GSDME pathway: a potential antibody-drug conjugate payload

doi: 10.21037/tcr-2026-0547

Figure Lengend Snippet: ΔA146 ply-CPP activates the Caspase-1/GSDME pyroptotic pathway in breast cancer cells. MDA-MB-231 and MDA-MB-453 cells were treated with 2µm ΔA146 ply-CPP for 24 h. (A) Western blotting was performed to detect the expression of full-length GSDME and its cleaved N-terminal fragment, with β-actin as the loading control. (B) Caspase-1 activity was measured using the Ac-YVAD-pNA substrate and presented as fold change relative to the PBS control. Data are shown as mean ± SD (n=3). (C) The levels of IL-1β, IL-18 and HMGB1 in the cell supernatants were quantified by ELISA. Data are presented as mean ± SD (n=3). CPP, cell-penetrating peptide; ELISA, enzyme-linked immunosorbent assay; GSDME, gasdermin E; HMGB, high mobility group box; IL-18, interleukin-18; IL-1β, interleukin-1β; M, protein molecular weight marker; PBS, phosphate-buffered saline; SD, standard deviation.

Article Snippet: Kits and assays comprised the CytoTox 96 ® Non-Radioactive Cytotoxicity Assay for LDH release (Promega, G1780, Madison, WI, USA), fluorescein isothiocyanate (FITC) protein labeling kit (Beyotime, P0639S, Shanghai, China), SYTOX Green nucleic acid stain (Beyotime, C1186), Caspase-1 activity assay kit (Beyotime, C1101), IL-1β ELISA kit (Multi Sciences, EK101B, Hangzhou, China), IL-18 ELISA kit (Multi Sciences, EK118), HMGB1 ELISA kit (Solarbio, SEKH-0409, Beijing, China), Isopropyl β-D-1-thiogalactopyranoside (IPTG) (Beyotime, ST098), high-glucose Dulbecco’s Modified Eagle Medium (DMEM) medium (Gibco, C11965500BT, Grand Island, NY, USA), fetal bovine serum (FBS) (Gibco, A5256701), 4’,6-Diamidino-2-phenylindole (DAPI) (Beyotime, C1002), Nickel-nitrilotriacetic acid (Ni-NTA) affinity chromatography resin (Qiagen, 30210, Hilden, Germany), and enhanced chemiluminescence (ECL) substrate (Bio-Rad, 1705060, Hercules, CA, USA).

Techniques: Western Blot, Expressing, Control, Activity Assay, Enzyme-linked Immunosorbent Assay, Peptide ELISA, Molecular Weight, Marker, Saline, Standard Deviation

Journal: Translational Cancer Research

Article Title: A pneumolysin mutant (ΔA146 ply) induces pyroptosis in triple-negative and HER2-positive breast cancer cells via the Caspase-1/GSDME pathway: a potential antibody-drug conjugate payload

doi: 10.21037/tcr-2026-0547

Figure Lengend Snippet: Schematic illustration of ΔA146 ply-induced pyroptosis in breast cancer cells via the Caspase-1/GSDME pathway. Upon cellular entry, ΔA146 ply triggers Caspase-1 activation, which cleaves GSDME to generate its pore‑forming N‑terminal fragment (GSDME‑NT). Membrane pore formation leads to pyroptotic cell death and release of IL‑1β, IL‑18, and HMGB1. This canonical pathway (10, 12) is supported by our data showing Caspase‑1 activation, GSDME cleavage, and inflammatory factor release. CPP, cell-penetrating peptide; GSDME, gasdermin E; IL-18, interleukin-18; IL-1β, interleukin-1β; HMGB1, high mobility group box 1.

Article Snippet: Kits and assays comprised the CytoTox 96 ® Non-Radioactive Cytotoxicity Assay for LDH release (Promega, G1780, Madison, WI, USA), fluorescein isothiocyanate (FITC) protein labeling kit (Beyotime, P0639S, Shanghai, China), SYTOX Green nucleic acid stain (Beyotime, C1186), Caspase-1 activity assay kit (Beyotime, C1101), IL-1β ELISA kit (Multi Sciences, EK101B, Hangzhou, China), IL-18 ELISA kit (Multi Sciences, EK118), HMGB1 ELISA kit (Solarbio, SEKH-0409, Beijing, China), Isopropyl β-D-1-thiogalactopyranoside (IPTG) (Beyotime, ST098), high-glucose Dulbecco’s Modified Eagle Medium (DMEM) medium (Gibco, C11965500BT, Grand Island, NY, USA), fetal bovine serum (FBS) (Gibco, A5256701), 4’,6-Diamidino-2-phenylindole (DAPI) (Beyotime, C1002), Nickel-nitrilotriacetic acid (Ni-NTA) affinity chromatography resin (Qiagen, 30210, Hilden, Germany), and enhanced chemiluminescence (ECL) substrate (Bio-Rad, 1705060, Hercules, CA, USA).

Techniques: Activation Assay, Membrane

Journal: Polymers

Article Title: Electrospun Cellulose Acetate Nanofibers for Healthcare Products: Towards Sensing Pads for Endometriosis

doi: 10.3390/polym18091036

Figure Lengend Snippet: IL-18 ( a ) and TNF-a ( b ) levels after direct contact with different types of electrospun membranes (n = 2 per group). No significant differences were detected through one-way ANOVA ( p > 0.05), indicating the absence of an early inflammatory response.

Article Snippet: After the exposure period, the release of the proinflammatory cytokines interleukin 18 (IL-18) (OriGene Human IL-18 ELISA Kit, OriGene, Rockville, MD, USA) and tumor necrosis factor-alpha (TNF-a) (Abnova TNF-α (human) ELISA Kit, Abnova, Taipei City, Taiwan) into the cell culture supernatant was quantified using an enzyme-linked immunosorbent assay (ELISA), according to the manufacturer’s protocol.

Techniques: